iga detection antibody 179 Search Results


15-pgdh/hpgd antibody  (Bio-Techne corporation)
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stem cell marker cd34 pe  (Miltenyi Biotec)
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tpx2 antibody  (Bio-Techne corporation)
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Bio-Techne corporation tpx2 antibody
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aldehyde dehydrogenase 2  (Santa Cruz Biotechnology)
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polyclonal goat antibody to gata4 #sc-1237  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology polyclonal goat antibody to gata4 #sc-1237
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polyclonal anti-rhoa primary antibody sc-179  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology polyclonal anti-rhoa primary antibody sc-179
Polyclonal Anti Rhoa Primary Antibody Sc 179, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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178 secondary antibody  (Abcam)
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Abcam 178 secondary antibody
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anti goat igg  (Santa Cruz Biotechnology)
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rhoa/c (rabbit polyclonal, sc-179  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology rhoa/c (rabbit polyclonal, sc-179
RhoC acts redundantly with RhoA to regulate apical junction formation. (A) 16HBE cells were seeded at low density and transfected with the indicated siRNAs. At 3 days posttransfection, cells were fixed and stained with anti-ZO-1 antibody. Scale bar shows 20 μm for all images. (B) Quantification of tight junction formation from 3 independent experiments (see Materials and Methods). Error bars, SEM; nsd, no significant difference; ***, P < 0.001. (C) RhoA expression is approximately 4-fold higher than RhoC expression in 16HBE cells. Left: lysates from HEK293T cells transfected with HA-tagged Rho GTPases were analyzed by Western blot assay with the <t>indicated</t> <t>antibodies.</t> Note that the <t>anti-RhoA/C</t> antibody binds with greater affinity (approximately 2-fold) to RhoC than to RhoA and does not recognize RhoB. Right: lysates from 16HBE cells transfected with the indicated siRNAs were analyzed by Western blot assay with the indicated antibodies. Note that endogenous RhoA (second lane, lower band) is recognized more strongly (approximately 2-fold) than RhoC (third lane, upper band) with the anti-RhoA/C antibody. (D) Expression of mouse RhoA or mouse RhoC rescues apical junction formation after depletion of endogenous RhoA. 16HBE cells seeded at low density were transfected with myc-tagged RhoA, myc-tagged RhoC, or a control plasmid. Six hours later, cells were transfected with RhoA siRNA duplex2 or siControl. Apical junction formation was analyzed at 3 days posttransfection. At least 100 myc-positive cells per condition from 3 independent experiments were analyzed. Error bars, SEM; nsd, no significant difference; **, P < 0.01.
Rhoa/C (Rabbit Polyclonal, Sc 179, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti hif 1α antibodies  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti hif 1α antibodies
RhoC acts redundantly with RhoA to regulate apical junction formation. (A) 16HBE cells were seeded at low density and transfected with the indicated siRNAs. At 3 days posttransfection, cells were fixed and stained with anti-ZO-1 antibody. Scale bar shows 20 μm for all images. (B) Quantification of tight junction formation from 3 independent experiments (see Materials and Methods). Error bars, SEM; nsd, no significant difference; ***, P < 0.001. (C) RhoA expression is approximately 4-fold higher than RhoC expression in 16HBE cells. Left: lysates from HEK293T cells transfected with HA-tagged Rho GTPases were analyzed by Western blot assay with the <t>indicated</t> <t>antibodies.</t> Note that the <t>anti-RhoA/C</t> antibody binds with greater affinity (approximately 2-fold) to RhoC than to RhoA and does not recognize RhoB. Right: lysates from 16HBE cells transfected with the indicated siRNAs were analyzed by Western blot assay with the indicated antibodies. Note that endogenous RhoA (second lane, lower band) is recognized more strongly (approximately 2-fold) than RhoC (third lane, upper band) with the anti-RhoA/C antibody. (D) Expression of mouse RhoA or mouse RhoC rescues apical junction formation after depletion of endogenous RhoA. 16HBE cells seeded at low density were transfected with myc-tagged RhoA, myc-tagged RhoC, or a control plasmid. Six hours later, cells were transfected with RhoA siRNA duplex2 or siControl. Apical junction formation was analyzed at 3 days posttransfection. At least 100 myc-positive cells per condition from 3 independent experiments were analyzed. Error bars, SEM; nsd, no significant difference; **, P < 0.01.
Anti Hif 1α Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-granulin  (Millipore)
90
Millipore rabbit anti-granulin
RhoC acts redundantly with RhoA to regulate apical junction formation. (A) 16HBE cells were seeded at low density and transfected with the indicated siRNAs. At 3 days posttransfection, cells were fixed and stained with anti-ZO-1 antibody. Scale bar shows 20 μm for all images. (B) Quantification of tight junction formation from 3 independent experiments (see Materials and Methods). Error bars, SEM; nsd, no significant difference; ***, P < 0.001. (C) RhoA expression is approximately 4-fold higher than RhoC expression in 16HBE cells. Left: lysates from HEK293T cells transfected with HA-tagged Rho GTPases were analyzed by Western blot assay with the <t>indicated</t> <t>antibodies.</t> Note that the <t>anti-RhoA/C</t> antibody binds with greater affinity (approximately 2-fold) to RhoC than to RhoA and does not recognize RhoB. Right: lysates from 16HBE cells transfected with the indicated siRNAs were analyzed by Western blot assay with the indicated antibodies. Note that endogenous RhoA (second lane, lower band) is recognized more strongly (approximately 2-fold) than RhoC (third lane, upper band) with the anti-RhoA/C antibody. (D) Expression of mouse RhoA or mouse RhoC rescues apical junction formation after depletion of endogenous RhoA. 16HBE cells seeded at low density were transfected with myc-tagged RhoA, myc-tagged RhoC, or a control plasmid. Six hours later, cells were transfected with RhoA siRNA duplex2 or siControl. Apical junction formation was analyzed at 3 days posttransfection. At least 100 myc-positive cells per condition from 3 independent experiments were analyzed. Error bars, SEM; nsd, no significant difference; **, P < 0.01.
Rabbit Anti Granulin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho perk thr980  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phospho perk thr980
RhoC acts redundantly with RhoA to regulate apical junction formation. (A) 16HBE cells were seeded at low density and transfected with the indicated siRNAs. At 3 days posttransfection, cells were fixed and stained with anti-ZO-1 antibody. Scale bar shows 20 μm for all images. (B) Quantification of tight junction formation from 3 independent experiments (see Materials and Methods). Error bars, SEM; nsd, no significant difference; ***, P < 0.001. (C) RhoA expression is approximately 4-fold higher than RhoC expression in 16HBE cells. Left: lysates from HEK293T cells transfected with HA-tagged Rho GTPases were analyzed by Western blot assay with the <t>indicated</t> <t>antibodies.</t> Note that the <t>anti-RhoA/C</t> antibody binds with greater affinity (approximately 2-fold) to RhoC than to RhoA and does not recognize RhoB. Right: lysates from 16HBE cells transfected with the indicated siRNAs were analyzed by Western blot assay with the indicated antibodies. Note that endogenous RhoA (second lane, lower band) is recognized more strongly (approximately 2-fold) than RhoC (third lane, upper band) with the anti-RhoA/C antibody. (D) Expression of mouse RhoA or mouse RhoC rescues apical junction formation after depletion of endogenous RhoA. 16HBE cells seeded at low density were transfected with myc-tagged RhoA, myc-tagged RhoC, or a control plasmid. Six hours later, cells were transfected with RhoA siRNA duplex2 or siControl. Apical junction formation was analyzed at 3 days posttransfection. At least 100 myc-positive cells per condition from 3 independent experiments were analyzed. Error bars, SEM; nsd, no significant difference; **, P < 0.01.
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Image Search Results


RhoC acts redundantly with RhoA to regulate apical junction formation. (A) 16HBE cells were seeded at low density and transfected with the indicated siRNAs. At 3 days posttransfection, cells were fixed and stained with anti-ZO-1 antibody. Scale bar shows 20 μm for all images. (B) Quantification of tight junction formation from 3 independent experiments (see Materials and Methods). Error bars, SEM; nsd, no significant difference; ***, P < 0.001. (C) RhoA expression is approximately 4-fold higher than RhoC expression in 16HBE cells. Left: lysates from HEK293T cells transfected with HA-tagged Rho GTPases were analyzed by Western blot assay with the indicated antibodies. Note that the anti-RhoA/C antibody binds with greater affinity (approximately 2-fold) to RhoC than to RhoA and does not recognize RhoB. Right: lysates from 16HBE cells transfected with the indicated siRNAs were analyzed by Western blot assay with the indicated antibodies. Note that endogenous RhoA (second lane, lower band) is recognized more strongly (approximately 2-fold) than RhoC (third lane, upper band) with the anti-RhoA/C antibody. (D) Expression of mouse RhoA or mouse RhoC rescues apical junction formation after depletion of endogenous RhoA. 16HBE cells seeded at low density were transfected with myc-tagged RhoA, myc-tagged RhoC, or a control plasmid. Six hours later, cells were transfected with RhoA siRNA duplex2 or siControl. Apical junction formation was analyzed at 3 days posttransfection. At least 100 myc-positive cells per condition from 3 independent experiments were analyzed. Error bars, SEM; nsd, no significant difference; **, P < 0.01.

Journal: Molecular and Cellular Biology

Article Title: The Rho Target PRK2 Regulates Apical Junction Formation in Human Bronchial Epithelial Cells ▿

doi: 10.1128/MCB.01001-10

Figure Lengend Snippet: RhoC acts redundantly with RhoA to regulate apical junction formation. (A) 16HBE cells were seeded at low density and transfected with the indicated siRNAs. At 3 days posttransfection, cells were fixed and stained with anti-ZO-1 antibody. Scale bar shows 20 μm for all images. (B) Quantification of tight junction formation from 3 independent experiments (see Materials and Methods). Error bars, SEM; nsd, no significant difference; ***, P < 0.001. (C) RhoA expression is approximately 4-fold higher than RhoC expression in 16HBE cells. Left: lysates from HEK293T cells transfected with HA-tagged Rho GTPases were analyzed by Western blot assay with the indicated antibodies. Note that the anti-RhoA/C antibody binds with greater affinity (approximately 2-fold) to RhoC than to RhoA and does not recognize RhoB. Right: lysates from 16HBE cells transfected with the indicated siRNAs were analyzed by Western blot assay with the indicated antibodies. Note that endogenous RhoA (second lane, lower band) is recognized more strongly (approximately 2-fold) than RhoC (third lane, upper band) with the anti-RhoA/C antibody. (D) Expression of mouse RhoA or mouse RhoC rescues apical junction formation after depletion of endogenous RhoA. 16HBE cells seeded at low density were transfected with myc-tagged RhoA, myc-tagged RhoC, or a control plasmid. Six hours later, cells were transfected with RhoA siRNA duplex2 or siControl. Apical junction formation was analyzed at 3 days posttransfection. At least 100 myc-positive cells per condition from 3 independent experiments were analyzed. Error bars, SEM; nsd, no significant difference; **, P < 0.01.

Article Snippet: The primary antibodies used were RhoA (clone 26C4) and RhoA/C (rabbit polyclonal, sc-179) from Santa Cruz Biotechnology (Santa Cruz, CA); occludin (rabbit polyclonal), ZO-1 (clone 1A12), ZO-1 (rabbit polyclonal), and E-cadherin (clone ECCD-2) from Invitrogen (Carlsbad, CA); E-cadherin (clone 34) and PRK2 (clone 22) from BD Transduction (Lexington, KY); phospho-PRK1 (Thr774)/PRK2 (Thr816) (rabbit polyclonal) from Cell Signaling (Beverly, MA); α-tubulin (clone YL1/2) from AbD Setotec (Raleigh, NC); β-actin (clone AC-74) and FLAG (clone M2) from Sigma-Aldrich; hemagglutinin (HA; clone 3F10) from Roche; and myc (clone 9E10) from Cancer Research UK (London, United Kingdom).

Techniques: Transfection, Staining, Expressing, Western Blot, Plasmid Preparation